Search results for "Candidatus liberibacter"
showing 6 items of 6 documents
Collecting and preserving plant DNA for huanglongbing diagnosis in citrus samples from China
2016
Accurate and sensitive detection of Citrus Huanglongbing associated ‘Ca. Liberibacter’species, not currently reported in the European and Mediterranean area, is an imperative need to define certification programs, to prevent introduction of the bacteria and/or their vectors in the unaffected areas, and to delineate efficient management strategies in those areas where the disease has spread. In this study, we compared different citrus sample preparation procedures for PCR based detection methods of ‘Candidatus Liberibacter asiaticus’, in order to find out the best a way to transport and preserve samples of Shatangju mandarin and fingered citron obtained during a survey in citrus orchards in …
Proteomic analysis highlights the role of detoxification pathways in increased tolerance to Huanglongbing disease.
2016
Background Huanglongbing (HLB) disease is still the greatest threat to citriculture worldwide. Although there is not any resistance source in the Citrus germplasm, a certain level of moderated tolerance is present. A large-scale analysis of proteomic responses of Citrus may help: 1) clarifying physiological and molecular effects of disease progression, 2) validating previous data at transcriptomic level, and 3) identifying biomarkers for development of early diagnostics, short-term therapeutics and long-term genetic resistance. Results In this work we have conducted a proteomic analysis of mature leaves of two Citrus genotypes with well-known differing tolerances to HLB: Navel orange (highl…
Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain
2020
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Transcriptome profiling of citrus fruit response to huanglongbing disease.
2010
Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB- affected fruit. It was followed by pathway and protein-protein network analysis and quantitative real time PCR analysis of highly regulated genes. We identified differentially regulated pathways and constructed networks that provide a deep insight into the metabolism of affected fruit. Data mining revealed that HLB enhanced transcription of genes involved in the light reactions of phot…
Genetic Mechanisms of the Devious Intruder Candidatus Liberibacter in Citrus.
2017
HLB symptom progression may result from three types of dysfunction occurring in Ca.L.-infected citrus: (1) a carbohydrate disorder linked to disruption of the source-sink relationship, (2) perturbation of hormonal crosstalk involved in plant immune responses (JA-SA signaling crosstalk), and (3) changes in the rapid activation of detoxifying pathways (particularly GSTs). The development of innovative short- or long-term biotechnological tools that allow beneficial modulation of these three pathways will help increase Citrus tolerance to this devastating disease.
Maladie du huanglongbing
2019
Maladie du huanglongbing. Analyse du risque phytosanitaire pour l’Union européenne